FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n01_t3r-r.3510000005266b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n01_t3r-r.3510000005266b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12815767
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC153870.1200630442173301TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT39700.019.38218543
CATCTCG39250.018.85920740
CGTATGC46800.016.73017745
GGCTACA126650.015.97061445
GTGACTG32450.015.9014731
TCGTATG50600.015.3848344
GACGTGT267050.015.3397371
CGGCTAC138300.014.56022344
ATCTCGT53900.014.10896241
TGACTGG38150.013.6233712
TCTCGTA61050.013.15678242
ACGGCTA155050.013.0598443
CACGGCT159250.012.67301942
GACTGGA40900.012.5973553
ACGTGTG328550.012.4839592
GTTCAGA116650.012.4785561
TCACGGC164800.012.25988141
GTCACGG169050.011.95166240
ACATCTC71750.011.91595545
GGAGTTC43750.011.7767277
Produced by FastQC (version 0.11.2)