FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n01_t2wt.35100000052685.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n01_t2wt.35100000052685.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14612811
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC318840.21819210554355353TruSeq Adapter, Index 14 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT67000.023.54011543
CATCTCG68000.022.53219840
CGTATGC80250.019.59735745
TCTCGTA84250.018.69362442
TCGTATG85550.018.46215844
GACGTGT533450.018.0965081
AGTTCAG103350.017.7699491
AGTTCCA307850.017.75957345
ATCTCGT87600.017.4393841
GTTCAGA161950.016.641381
TTCAGAC249850.016.4507061
TCAGACG251450.016.3743782
GTGACTG29050.015.281261
CAGACGT271350.015.1984053
CAGTTCC365550.014.925549544
ACGTGTG671550.014.3929992
ACAGTTC397800.013.79470443
GACTGGA32400.013.6800163
TGACTGG32950.013.451672
GTTCCAT188750.013.13590645
Produced by FastQC (version 0.11.2)