FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n01_t2r-r.35100000052642.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n01_t2r-r.35100000052642.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11158563
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG67450.021.0803851
TGACTGG74400.019.112222
GACTGGA75900.018.7345093
GGAGTTC86950.016.6641547
CTGGAGT89700.016.0027735
ACTGGAG94800.015.0706644
TGGAGTT103300.014.0266046
CATCTCG26300.013.94436940
GAGTTCA105350.013.8177328
CTCGTAT30400.012.43376543
CAGATCA74500.011.92910445
AGTTCAG129800.011.162939
ATCTCGT37400.010.287065541
TCTCGTA40500.09.83298242
TGCCCCG13800.09.7822595
CCCCGGG13800.09.6192227
GTTCAGA152550.09.5571810
GTGCCCC15000.09.4496624
GTGTGAC15500.09.289995
GAGTGTG13350.09.2693323
Produced by FastQC (version 0.11.2)