FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n01_t2p.351000000526af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n01_t2p.351000000526af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14324457
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC225070.15712288430898289TruSeq Adapter, Index 15 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT49450.020.33796743
GACGTGT432450.017.4553131
GTTCAGA136950.016.7825071
CGTATGC60600.016.78155345
TTCAGAC211050.016.3245351
TCAGACG211400.016.2624032
AGTTCAG90650.015.9334161
AATCTCG62850.015.50061640
TCGTATG66400.015.2818144
CAGACGT226750.015.14166453
ATGTCAA284350.015.12867845
TCTCGTA68300.014.9555242
CATGTCA294950.014.50107144
ATCTCGT68350.014.48373341
GTGACTG25450.014.3431951
ACGTGTG550900.013.7143192
ACATGTC322600.013.44649243
CACATGT320950.013.32634542
AGACGTG263050.013.0265054
TCACATG337600.012.6557841
Produced by FastQC (version 0.11.2)