FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n01_t1r-r.35100000052635.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n01_t1r-r.35100000052635.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12613810
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG128750.026.9178711
TGACTGG136750.025.3701842
GACTGGA142350.024.482773
ACTGGAG162600.021.4337184
CTGGAGT164200.021.211165
GGAGTTC168150.020.9403577
TGGAGTT184250.019.0250846
GAGTTCA186850.018.8205578
AGTTCAG212550.016.3649629
TATCTCG32250.015.83662940
CTCGTAT34800.015.06410343
GTTCAGA239600.014.51741510
TCAGACG249650.013.77978812
GCCAATA126650.013.62563645
CAGACGT254450.013.45794713
TTCAGAC260350.013.33444511
AGACGTG263200.012.98489714
GTGTGAC16300.012.8369535
CGCCAAT151100.011.37615444
ATCTCGT45050.011.33698841
Produced by FastQC (version 0.11.2)