FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n01_t0r-r.3510000005261b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n01_t0r-r.3510000005261b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14049066
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC171960.12239959581654752TruSeq Adapter, Index 5 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGA330700.021.50580645
CTCGTAT46950.019.79155343
GTGACTG63700.018.9966831
GACTGGA67550.017.9859683
TGACTGG67100.017.8383452
ATCTCGT54500.016.55439441
CACAGTG491200.015.96740845
GGAGTTC76350.015.8834617
CAGTGAT168400.015.65852845
TCTCGTA58700.015.52323942
CTGGAGT82600.014.572675
CGTATGC64350.014.26517245
TCGTATG66000.014.1812644
ACTGGAG86850.013.96318154
GATCTCG69150.012.81945140
TGGAGTT97500.012.59950356
GAGTTCA98850.012.26810558
ACACAGT647150.012.19955144
TGATCTC86500.011.70474145
GTGATCT84200.011.70381544
Produced by FastQC (version 0.11.2)