FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n01_p39550.351000000526c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n01_p39550.351000000526c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9874023
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGTGCCCCGGGTAACCCAGTTCCTCACTATTTTTTACTGCGGAAGCGGA151500.15343290166530907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCCG39750.018.3389975
GTGCCCC40300.018.0328834
GTGACTG20950.017.7473891
CCCCGGG42100.017.3687677
CCCGGGT43000.017.0575588
CCGGGTA47500.015.9626149
CAGGTGC46400.015.8319421
TGACTGG25100.015.0592472
GCCCCGG49450.014.9236576
GACTGGA25600.014.7651223
GGAGTTC26100.014.5684697
GGGTAAC53250.014.28120211
GGTGCCC51450.014.2560753
GTAACCC53300.013.97231913
CGGGTAA56750.013.83653210
GGTAACC56300.013.42760612
TAACTAG21450.013.42611630
CTGGAGT29000.013.1116225
AGGTGCC58450.012.7027322
ACCCAGT58700.012.61030216
Produced by FastQC (version 0.11.2)