FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n01_p39532.351000000526e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n01_p39532.351000000526e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10538797
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC327380.31064266633089144TruSeq Adapter, Index 21 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT66250.024.8595143
CGTATGC71400.023.53909945
TCGTATG74850.022.51424844
GACGTGT234300.020.3494221
TCGATCT177950.018.53546144
TCTCGTA90200.018.30867642
ATCTCGT89450.017.8836741
GTGCCCC27650.017.2507574
GTTTCGA442350.017.1916745
TGCCCCG28000.016.954775
CCCCGGG29150.016.3630687
TTCGATC207100.016.06776643
CCCGGGT29800.015.9306558
TCAGACG98950.015.8938382
TTCAGAC101350.015.6070941
ACGTGTG308000.015.479172
GTGACTG17600.015.2350191
CCGGGTA33450.015.2012659
CAGACGT105600.014.9781713
CGTTTCG538500.014.08028944
Produced by FastQC (version 0.11.2)