FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n01_p39523.3510000005270d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n01_p39523.3510000005270d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12632426
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC661900.5239690301767848TruSeq Adapter, Index 22 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT121100.027.38541243
CGTATGC122200.027.34142745
TCGTATG130000.025.71824844
TCTCGTA147300.021.9034442
ATCTCGT152350.020.85250341
AGTTCAG94500.020.816311
GACGTGT514100.018.864531
GTTCAGA158000.018.539911
ACGATCT266350.016.7677144
TACGATC279600.016.00529143
GATCTCG203900.015.52540140
TTCAGAC231750.015.148481
GTGCCCC28600.015.1043194
TCAGACG235950.014.951752
CCCCGGG28550.014.8155467
ACGTGTG656300.014.7583132
CGTACGA529050.014.69748245
TGCCCCG28800.014.6869395
CCCGGGT29250.014.4609868
CCGGGTA32700.014.1049669
Produced by FastQC (version 0.11.2)