FastQCFastQC Report
Sun 24 Apr 2016
HJ533BCXX_l01n01_p39514.35100000052727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ533BCXX_l01n01_p39514.35100000052727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10674332
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGC865740.8110484103361222TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC146050.029.76274145
CTCGTAT149450.028.57377843
TCGTATG156500.027.76101144
TCTCGTA176200.023.456942
ATCTCGT184950.022.20117241
GACGTGT455950.021.3383981
GTTCAGA107500.018.8428961
TTCAGAC170800.017.914651
TCAGACG174300.017.5811422
GATCTCG244400.016.73632440
AGTTCAG65300.016.734971
GGATCTC298150.016.06601745
CAGACGT192250.015.8694093
ACGTGTG619500.015.7040522
GTAGGGA16100.015.3721352
TGGATCT317350.015.19326344
GTGGATC322250.014.94129443
TGTAGGG16450.014.9299041
TGCCCCG19300.014.6886065
GTGCCCC19550.014.5007734
Produced by FastQC (version 0.11.2)