FastQCFastQC Report
Fri 3 Jan 2025
HJ2TNDRX5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ2TNDRX5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920337993
Sequences flagged as poor quality0
Sequence length24
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCACACTAGGTGTAGATCTCGGT30999204833.6824134565528No Hit
GACTCTCCCGGTGTAGATCTCGGT14647898515.915781605682339No Hit
TGTGACGAACGTGTAGATCTCGGT11616356412.621837290596346No Hit
AGACCATCGGGTGTAGATCTCGGT773160138.40082812923708No Hit
AGATCTCGGTGGTCGCCGTATCAT85553290.9295855506423714Illumina Single End PCR Primer 1 (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGG29948880.3254117533752624No Hit
CAGACGCGAACCTAAGATTATCAC16413020.17833687324478412No Hit
CAGACGCGAGAAAGGCTTGACCGT10782940.11716282585326238No Hit
CAGACGCGCGCATCCACGCATGAT10522000.11432756313473195No Hit
CAGACGCGAGGTTGCGAAGTGAGA10324130.1121775921294602No Hit
CAGACGCGCCACTTAACCTCAGGA10182770.11064163467605537No Hit
CAGACGCGCGACAAACTGACCGAA9837880.10689420707203164No Hit
CAGACGCGGTGGCCAACCGGTTAG9782590.10629344951969184No Hit
CAGACGCGAACTGATGATGATGAC9626660.10459918066209833No Hit
CAGACGCGTCACGTTACTAAAGCT9210140.10007345203665846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)