FastQCFastQC Report
Fri 3 Jan 2025
HJ2TNDRX5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ2TNDRX5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920337993
Sequences flagged as poor quality0
Sequence length10
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGACGAAA30995168933.67802821979121No Hit
TCTACCATTT14827147416.110545813357508No Hit
CACGGTGAAT11647684412.655876958890255No Hit
TCGGCTCTAC765922158.322183326403216No Hit
ACGGGACTAT339139203.684941864613428No Hit
GAACATGAAT332199923.609542608549031No Hit
TGCATCTGAT325004653.5313618743543493No Hit
CTTTCGACAT319901383.4759119196766664No Hit
TAACGTCCAT288129393.130691030811329No Hit
CTCATCATAT276937733.0090872278050136No Hit
AGGTCATAAT232731192.5287578234315053No Hit
GGGGGGGGGG156456091.699985127094498No Hit
GCTGAGGGAT66368160.7211281127671538No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)