FastQCFastQC Report
Sun 30 Jun 2024
HJ2JWDSXC_n02_IFN_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ2JWDSXC_n02_IFN_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336196628
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72581942.158913384461429No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC14024940.4171648027356182Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGT7974970.23721148089563826Illumina Single End PCR Primer 1 (97% over 37bp)
GCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC4170200.12404050643839296Illumina Single End PCR Primer 1 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)