FastQCFastQC Report
Sun 30 Jun 2024
HJ2JWDSXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ2JWDSXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306773976
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGAGATCTCGTAT18019810.5873969570352343TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGTGGTATCTCGTAT13645020.4447906624256811TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAGCTTATCTCGTAT8954410.2918894919561234TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGTGTATCTCGTATG7240280.2360135007018979TruSeq Adapter, Index 25 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCAGACTCGGTGACTGGAGTTCAGACG5167840.16845757477159667Illumina PCR Primer Index 9 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTTGGTATCTCGTATG3707200.12084467034452753TruSeq Adapter, Index 8 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATGGGAGATGGTGACTGGAGTTCAGACG3476740.11333229908654312RNA PCR Primer, Index 14 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGCTTATCTCGTATG3237740.1055415469792001TruSeq Adapter, Index 5 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)