FastQCFastQC Report
Sun 30 Jun 2024
HJ2JWDSXC_n01_SAHA_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ2JWDSXC_n01_SAHA_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305897977
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGCTACCATCTCGTAT98668593.2255391476485635TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGC5598430.1830162479302699TruSeq Adapter, Index 4 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGGCTACCATCTCGTATGCCGT3913840.12794592623278447TruSeq Adapter, Index 10 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)