FastQCFastQC Report
Sun 30 Jun 2024
HJ2JWDSXC_n01_SAHA_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ2JWDSXC_n01_SAHA_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650257802
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAGCTTATCTCGTAT28728191644.1796953633476TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGA42566200.6546049869617712TruSeq Adapter, Index 15 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGGAGCTTATCTCGTATGCCGT13101990.2014891626013893TruSeq Adapter, Index 15 (96% over 31bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGGAGCTTATCTCGTATGCC11919680.18330698937157852TruSeq Adapter, Index 15 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAGCTTATCGCGTAT11707880.18004981968674633TruSeq Adapter, Index 15 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAGCTTATCTCGTA10572500.16258936021193637TruSeq Adapter, Index 15 (97% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGGAGCTTATCTCGTATGCCG10484220.1612317448211102TruSeq Adapter, Index 15 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAGCTTATCTCGTATG7403480.11385453549698432TruSeq Adapter, Index 15 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)