FastQCFastQC Report
Sun 30 Jun 2024
HJ2JWDSXC_n01_SAHA_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ2JWDSXC_n01_SAHA_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827890684
Sequences flagged as poor quality0
Sequence length151
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGTGGTATCTCGTAT47455555357.32104034643298TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTG37766310.4561750812019042TruSeq Adapter, Index 25 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCTGTGGTATCTCGTATGCC18719300.2261083541797651TruSeq Adapter, Index 25 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGTGGTATCGCGTAT18182620.21962585582132244TruSeq Adapter, Index 25 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGTGGTATCTCGTA15931290.19243228976834337TruSeq Adapter, Index 25 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTCTGTGGTATCTCGTATGCCGT15832280.19123635892972557TruSeq Adapter, Index 25 (96% over 32bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTCTGTGGTATCTCGTATGCCG15139220.18286496384829473TruSeq Adapter, Index 25 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGTGGTATCTCGTATG13356410.16133059905285757TruSeq Adapter, Index 25 (97% over 36bp)
GATCGGAAGAGCACACGTCTGATCGGAAGAGCACACGTCTGAACTCCAGT10066970.12159781713403119Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GGTCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGTGGTATCTCGTAT9675010.1168633756482758TruSeq Adapter, Index 3 (97% over 34bp)
GNTCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGTGGTATCTCGTAT8543330.10319393810209851TruSeq Adapter, Index 3 (97% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)