FastQCFastQC Report
Sun 30 Jun 2024
HJ2JWDSXC_n01_JQ1_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ2JWDSXC_n01_JQ1_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357034067
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGGCTGAATCTCGTAT161954284.536101592792825TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGG5110060.14312527773435132TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGGCTGAATCTCGGAT4684760.13121324918274535TruSeq Adapter, Index 20 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)