FastQCFastQC Report
Sun 30 Jun 2024
HJ2JWDSXC_n01_JQ1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ2JWDSXC_n01_JQ1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410011550
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATAACAATCTCGTAT5864756514.303881195541932TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAT30387370.7411344875528507TruSeq Adapter, Index 2 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGATAACAATCTCGTATGCCGT7741050.18880077890488695TruSeq Adapter, Index 2 (96% over 32bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGATAACAATCTCGTATGCCG4248020.10360732520827767TruSeq Adapter, Index 2 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)