FastQCFastQC Report
Sun 30 Jun 2024
HJ2JWDSXC_n01_JQ1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ2JWDSXC_n01_JQ1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711331339
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGAGATCTCGTAT34726116348.81848218415132TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGGAGATCTCGTAT117367221.6499655443973065TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATG73869161.0384634550735012TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGGTGAGATCTCGTAT16627670.23375421675327032TruSeq Adapter, Index 20 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGAGATCTCGTA13577840.19087926055792687TruSeq Adapter, Index 9 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGAGATCTCGTATGCC12271170.1725099025898534TruSeq Adapter, Index 9 (96% over 33bp)
GAAGAGCACACGTCTGAACTCCAGTCACGATGTGAGATCTCGTATGCCGT11715470.16469779071550228TruSeq Adapter, Index 9 (96% over 31bp)
GATCGGAAGAGCAGATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAAC11351810.15958540524811604Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATCGGAAGAGCAGATCGGAAGAGCAGATCGGAAGAGCAGATCGGAAGAG10213370.14358104922465675No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGATGTGAGATCTCGTATGCCG9917840.13942644526167852TruSeq Adapter, Index 9 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGAGATCGCGTAT9236500.129848067891748TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGATCGGAAGAGCACACGTCTGAACTCCAGT7316930.10286247208349131Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)