FastQCFastQC Report
Sun 30 Jun 2024
HJ2JWDSXC_n01_IFN_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ2JWDSXC_n01_IFN_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268225953
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTTTCGATCTCGTAT92673803.4550646185978877TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT4861150.1812333946670701TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACGAGTTTCGATCTCGTATGCCGT4739020.17668014399784795TruSeq Adapter, Index 7 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)