FastQCFastQC Report
Sun 30 Jun 2024
HJ2JWDSXC_n01_IFN_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ2JWDSXC_n01_IFN_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336196628
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTTAATCATCTCGTAT80293282.388283323293772TruSeq Adapter, Index 21 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTTAATCATCTCGTA12322870.3665375846660782TruSeq Adapter, Index 21 (97% over 37bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG9374810.2788490192709488Illumina Single End PCR Primer 1 (96% over 32bp)
GAAGAGCACACGTCTGAACTCCAGTCACCTTTAATCATCTCGTATGCCGT5004280.14884979750599997TruSeq Adapter, Index 21 (96% over 32bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTT4890690.14547111995424297TruSeq Adapter, Index 21 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)