FastQCFastQC Report
Sun 30 Jun 2024
HJ2JWDSXC_n01_IFN_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ2JWDSXC_n01_IFN_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380055552
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAATGTCGATCTCGTAT6692587717.609498571408842TruSeq Adapter, Index 5 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACAAATGTCGATCTCGTATGCCGT9589160.2523094308065785TruSeq Adapter, Index 5 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)