FastQCFastQC Report
Sun 30 Jun 2024
HJ2JWDSXC_n01_DMS0_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ2JWDSXC_n01_DMS0_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317078896
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTGACATCTCGTAT134663374.246998828960222TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACC18147080.5723206504415229TruSeq Adapter, Index 1 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACAACCTGACATCTCGTATGCCGT6199610.19552263106151346TruSeq Adapter, Index 1 (96% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTGACATCTCGTA5821810.18360761543713713TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCAGATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAAC4661750.14702176836139863Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)