FastQCFastQC Report
Sun 30 Jun 2024
HJ2JWDSXC_n01_DMS0_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ2JWDSXC_n01_DMS0_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343442207
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATATAAATCTCGTAT307809168.96247326992049TruSeq Adapter, Index 2 (97% over 40bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT46142711.343536381362702TruSeq Adapter, Index 2 (100% over 37bp)
GATCGGAAGAGCAGATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAAC8661080.2521844963569082Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAAGAGCACACGTCTGAACTCCAGTCACCGATATAAATCTCGTATGCCGT5669950.1650918228579867TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCAGATCGGAAGAGCAGATCGGAAGAGCAGATCGGAAGAG5612960.16343244614660887No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGATATAAATCTCGTATGCCG4737250.1379344152653899TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)