FastQCFastQC Report
Sun 30 Jun 2024
HJ2JWDSXC_n01_DMS0_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ2JWDSXC_n01_DMS0_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427565306
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCGAGAAATCTCGTAT4491322010.504411693309839TruSeq Adapter, Index 11 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACCGCGAGAAATCTCGTATGCCGT14248410.3332452329516184TruSeq Adapter, Index 11 (96% over 31bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCG4359940.10197132318308351TruSeq Adapter, Index 11 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)