FastQCFastQC Report
Sun 30 Jun 2024
HJ2JWDSXC_n01_DMS0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ2JWDSXC_n01_DMS0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318906132
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGGATTCATCTCGTAT4742503014.871156506956096TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGG13332080.4180565584107364TruSeq Adapter, Index 10 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGGGATTCATCTCGTATGCCGT5591700.17533999628454933TruSeq Adapter, Index 10 (96% over 31bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGGGATTCATCTCGTATGCC3289660.10315449186784531TruSeq Adapter, Index 10 (96% over 33bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGGGATTCATCTCGTATGCCG3256060.10210089030210306TruSeq Adapter, Index 10 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)