FastQCFastQC Report
Sun 30 Jun 2024
HJ2JWDSXC_n01_DMS0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ2JWDSXC_n01_DMS0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396544792
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACATTATCTCGTAT6006265515.146499515747013TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATA20879390.5265329521715166TruSeq Adapter, Index 7 (97% over 37bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT13116430.33076792999465243Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAAGAGCACACGTCTGAACTCCAGTCACCATACATTATCTCGTATGCCGT7789180.1964262337355322TruSeq Adapter, Index 22 (96% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACATTATCTCGTA4660950.11753905470532569TruSeq Adapter, Index 22 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)