FastQCFastQC Report
Thu 31 May 2018
HHYCTBCX2_l02n02.332000000df916.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHYCTBCX2_l02n02.332000000df916.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187808211
Sequences flagged as poor quality0
Sequence length99
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT8824850.079.504031
AGCAGTG9219650.075.79182
GCAGTGG9577550.072.695813
CAGTGGT9684200.071.847194
TGGTATC9893700.070.009657
AGTGGTA10195450.068.170635
GGTATCA10211750.067.999238
GTGGTAT10237150.067.844626
GTATCAA10565500.065.924189
CATGGGG5815800.038.4161424-25
AGAGTAC9955150.037.83928318-19
GCAGAGT10013850.037.6060416-17
TACATGG9839450.037.57381422-23
CAACGCA10156150.037.3482712-13
ATCAACG10342350.036.70694410-11
ACGCAGA10517750.036.0761514-15
ACATGGG9656800.035.08030724-25
CAGAGTA9934400.034.99551418-19
TCAACGC10055550.034.73985312-13
AGTACAT10958100.034.1957620-21
Produced by FastQC (version 0.11.2)