FastQCFastQC Report
Thu 31 May 2018
HHYCTBCX2_l02n01.331000000df919.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHYCTBCX2_l02n01.331000000df919.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187808211
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTCTCTCTCTCTCTCTCTCTCTC6263280.33349340620682444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC571950.018.3796631
GGGAGAT564100.018.0470641
AAACCTG725900.018.0088331
ATGGGAG534750.017.9932941
CATGGCG549400.017.9718531
TGACGGA351300.017.95673210
CTGCGGA452850.017.9412171
GATGAAA550600.017.9269451
GCTGCAG683250.017.9208851
TGAGCCG433300.017.8607081
GGAAAGC456500.017.7377551
CGATTGA733800.017.679981
GGGAATG445950.017.6683061
ACTGATG459600.017.659381
CAATGGA426850.017.57958
TGACGGC375900.017.5523931
ATGACGG360600.017.5146799
CGCTGGA477250.017.416931
GCCGAAG328000.017.4037783
GACTGCG460450.017.320151
Produced by FastQC (version 0.11.2)