FastQCFastQC Report
Thu 31 May 2018
HHYCTBCX2_l01n02.332000000df923.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHYCTBCX2_l01n02.332000000df923.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184050267
Sequences flagged as poor quality0
Sequence length99
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT8680350.079.484031
AGCAGTG9071550.075.828882
GCAGTGG9429600.072.7981643
CAGTGGT9548600.071.8711554
TGGTATC9780350.070.109467
AGTGGTA10069050.068.2416155
GGTATCA10094500.068.166998
GTGGTAT10098400.068.0466846
GTATCAA10421750.066.1572959
CATGGGG5695850.038.4163824-25
AGAGTAC9784350.037.85181418-19
TACATGG9656650.037.67686522-23
GCAGAGT9864250.037.62131516-17
CAACGCA9994000.037.42819612-13
ATCAACG10205500.036.76510610-11
ACGCAGA10357300.036.07678214-15
ACATGGG9479850.035.1527524-25
CAGAGTA9769600.035.03508418-19
TCAACGC9913700.034.7910812-13
AGTACAT10775800.034.2441720-21
Produced by FastQC (version 0.11.2)