FastQCFastQC Report
Thu 31 May 2018
HHYCTBCX2_l01n01.331000000df926.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHYCTBCX2_l01n01.331000000df926.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184050267
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTCTCTCTCTCTCTCTCTCTCTC6284970.3414811672074347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC564350.018.19331
TGACGGA338050.018.11022810
GCTGCAG669050.018.091791
AAACCTG706150.018.0645661
GGGAGAT550300.018.0587161
CTGCGGA447500.018.0199411
CATGGCG544100.018.007031
ATGGGAG540900.017.9772531
GATGAAA544700.017.9253181
TGAGCCG419600.017.8651221
ACTGATG455900.017.808061
GGAAAGC438400.017.7164671
CGATTGA723150.017.6530081
TGACGGC371750.017.6316641
ATGACGG349800.017.5347949
CAATGGA415150.017.515128
GGGAATG438000.017.5138131
GAATGAA413650.017.3938521
TGGACGC436050.017.389231
GCCGAAG329800.017.3416733
Produced by FastQC (version 0.11.2)