FastQCFastQC Report
Sat 13 May 2023
HHWYGAFX5_n01_EN134_hs.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHWYGAFX5_n01_EN134_hs.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3274536
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT124750.3809700061321665TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTTT56120.17138306007324397TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)