FastQCFastQC Report
Sat 13 May 2023
HHWYGAFX5_n01_EN124_hs.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHWYGAFX5_n01_EN124_hs.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673803
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGGGGGGT22670.33644848716909836TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGGGGGTT18930.28094264940939706TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGGGGGGG15480.22974074024603633TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGGGGTT7970.11828383073390887TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)