FastQCFastQC Report
Thu 27 Jun 2019
HHWTNBGXB_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHWTNBGXB_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387683615
Sequences flagged as poor quality0
Sequence length99
%GC37

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32213970.8309345237610828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA3010150.037.223645
ATCAACG3304850.034.264943
TCAACGC3488650.032.5360684
CGCAGAG3667050.031.8639058
AACGCAG3660900.031.0616286
GCAGAGT3992000.029.2240459
CCCGATA435200.027.2679822
CCCCGAT236100.026.9270211
ACGCAGA4361150.026.2770967
GTATCAA4865950.026.1982461
CGATAGT1720450.025.4247174
GTGACTG4181950.024.5167859
TATCAAC4903650.024.0137272
GTCCTAT833250.023.0174581
ACTGTGC837650.022.9770588
TAGTCTG1516650.022.8820979
CTGTGCA679100.022.8592269
TAGTGAC4603100.022.5750087
CTACGCC794850.021.8884323
CAGAGTG3571200.021.87553810-11
Produced by FastQC (version 0.11.5)