FastQCFastQC Report
Thu 27 Jun 2019
HHWTNBGXB_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHWTNBGXB_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387683615
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCG33367085086.0678236298431Illumina Single End PCR Primer 1 (100% over 8bp)
ATATCTCG188529404.862970543648072Illumina PCR Primer Index 6 (100% over 8bp)
AGCTCTCG108763362.8054670301193925No Hit
TACTCCTT98430772.538945836026627ABI Solid3 GAPDH Reverse Primer (100% over 8bp)
AAATCTCG63560201.6394863631262826RNA PCR Primer, Index 13 (100% over 8bp)
ATCTCTCG28853700.744258949401305No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)