FastQCFastQC Report
Thu 27 Jun 2019
HHWTNBGXB_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHWTNBGXB_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387683615
Sequences flagged as poor quality0
Sequence length8
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGCGA4324635011.15506261465293No Hit
GCTACGCT3947412810.182047028219133No Hit
GGGGGGGG344799138.89382776726326No Hit
CGTACTAG320399378.264454766807724No Hit
CGAGGCTG296758417.654654427425312No Hit
CTCTCTAC274139697.071221980841259No Hit
CAGAGAGG269739916.957733047345836No Hit
TAGGCATG257642796.645697162104723No Hit
AGGCAGAA244540636.307737044806498No Hit
AAGAGGCA232927876.008194852392717No Hit
GTAGAGGA232065795.9859581633337795No Hit
GGACTCCT225649795.8204623891572No Hit
TCCTGAGC223316145.760267686319423No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)