FastQCFastQC Report
Thu 27 Jun 2019
HHWTNBGXB_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHWTNBGXB_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387683615
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTCACTCTGCGTTGATACCTGTC4900320.12639997695027683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTA182077050.020.9027658
GTCAGTC80006550.020.9012954
CTGTCCG112521600.020.8948977
AGTCACT182351850.020.8948767
TCACTAT181994150.020.880169
CACTATC181791800.020.87983510
TGTCCGC112516300.020.8785468
TCTGTCC113008450.020.874546
ACTATCG73750400.020.87304111
CAGTCAC182786150.020.8720196
TACTAGG27868650.020.8698186
GTCCGCA112440100.020.8661429
GTACTAG26620800.020.8495125
TTCTGTC113217850.020.8481085
TCAGTCA183112200.020.8300575
ACTATCC37057900.020.82936111
CTCAGTC33298400.020.828074
TCCGCAG112683650.020.81672910
ACTAGGT28005450.020.8119267
CTATCGG32721750.020.80778512
Produced by FastQC (version 0.11.5)