FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12845987
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3049682.374033229210025No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1106060.8610159733152462Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN526220.4096376557130254No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG75850.070.5165561
TGACTGG76600.068.296512
GGAGTTC62850.046.6772887
ACTGGAG116150.045.086454
TGGAGTT121100.042.401536
CGGAAGA891550.041.55995
AGAGCGT909350.041.3339359
TCGGAAG886550.041.2259834
AAGAGCG932150.040.393188
ATCGGAA896750.039.9203223
GATCGGA903450.039.6840172
AGATCGG916800.038.9728931
GATAGAT61450.037.9569748
GACTGGA141450.037.793413
GAAGAGC1032550.036.577657
GGTCGGA17350.035.916092
GGAAGAG1099700.034.489576
GAGTTCA88200.032.9331558
GGAGTTA72800.032.3376057
AGTTCAG91050.030.7087069
Produced by FastQC (version 0.11.5)