FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_pDZ-nonamp-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_pDZ-nonamp-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877530
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT22840.26027600196004697No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA20900.23816849566396592No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG19440.2215308878328946No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC19070.21731450776611627No Hit
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT18630.21230043417319067No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG18450.210249222248812No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG18160.20694449192620196No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT17580.20033503128098185No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC16030.18267181748772124No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG15870.18084851799938462No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG15080.17184597677572278No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT14860.16933893997925997No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC14210.1619317858078926No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA14130.1610201360637243No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG14110.16079222362768225No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT14010.15965266144747187No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT13390.15258737593016763No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT13230.15076407644183104No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT13180.15019429535172588No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT13120.14951055804359964No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA13110.14939660182557862No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT13090.14916868938953654No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA13040.14859890829943134No Hit
GTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAG12930.14734538990119997No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC12660.14426857201463197No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG12410.14141966656410607No Hit
CTATTGCAATGAAAATAAATTTCCTTTATTAGCCAGAAGTCAGATGCTCA12330.14050801681993777No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT12200.1390265859856643No Hit
GGCTAAAGCATGGAACCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAA12000.1367474616252436No Hit
ATTCTAGACGGGCCGGCCCCTGCGTGTGGCACGGGCGGCCGGGAGGGCGT11900.1356078994450332No Hit
CTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG11740.13378459995669664No Hit
GTGCTGGAGTACTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCAT11520.13127756316023384No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC11400.1299100885439814No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT11260.1283147014916869No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA11250.12820074527366587No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC11220.12785887661960274No Hit
GTTCTGAGGGGATTCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCC11160.12717513931147653No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT11110.12660535822137134No Hit
TAACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGA11050.12592162091324513No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCTGTGACATGGTGGAATAG11040.12580766469522411No Hit
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT10950.12478205873303477No Hit
CTCCTGGAGTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAAC10890.12409832142490855No Hit
GTATTGACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACGA10810.12318667168074025No Hit
CTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCT10610.12090754732031954No Hit
GTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT10590.12067963488427745No Hit
CCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAAATACGTCGGAGAGTT10540.12010985379417227No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC10450.11908424783198296No Hit
TCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCCAGCACTAAGCATC10430.11885633539594087No Hit
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA10290.11726094834364637No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT10260.11691907968958326No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA10140.11555160507333083No Hit
TTTCAGAAGGATGCGAAAGTGCTTTTTCAAAATTGGGGAGTTGAACCTAT10100.11509578020124668No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAG10090.11498182398322564No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG10050.1145259991111415No Hit
GAATGAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCA9870.11247478718676283No Hit
ATCCCAATCATTCCCATCACATTGTCGATAGGTTCAACTCCCCAATTTTG9770.11133522500655249No Hit
GTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC9720.1107654439164473No Hit
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTT9650.10996775039030006No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC9650.10996775039030006No Hit
GATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAGC9580.10917005686415279No Hit
GCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTA9560.10894214442811073No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG9550.10882818821008969No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGA9520.10848631955602658No Hit
GGCCAATACAGTGGGTTTGTAAGAACTCTGTTCCAACAAATGAGGGATGT9490.10814445090196345No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA9490.10814445090196345No Hit
ATGCAGGATCAGACCGAGTGATGGTATCACCTCTGGCTGTGACATGGTGG9460.10780258224790036No Hit
ATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAA9430.10746071359383726No Hit
GTGTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAG9430.10746071359383726No Hit
GAAGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAA9420.10734675737581621No Hit
CTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGATTATT9370.10677697628571102No Hit
ATGTACACACTGCTTGTTCCACCAGCCACTGGGAGGAATCTCGTTTTGCG9340.10643510763164793No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT9330.10632115141362689No Hit
GTGGAATAGGAATGGACCAATAACAAATACAGTTCATTATCCAAAAATCT9300.10597928275956378No Hit
GCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCT9260.10552345788747963No Hit
AGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAAGA9210.10495367679737445No Hit
GCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAA9080.10347224596310098No Hit
TCTCTGTTCCCTGTGTTTCACTGACCTCCTCGGGAGACAGTAGTACATTT9080.10347224596310098No Hit
CCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGG9030.10290246487299579No Hit
GTATTTTCACAAGAGGATTGTATGATAAAAGCAGTCAGAGGTGATCTGAA9020.10278850865497474No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG9010.10267455243695373No Hit
TAATAAAACTTCTTCCCTTCGCAGCCGCTCCACCAAAGCAAAGTAGAATG9000.10256059621893268No Hit
GTCCAAACTCATCAATGTATCTTATCATGTCTGGATCCGCTGCATTAATG8970.10221872756486959No Hit
CTCCAGCACGGAGAGGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCC8970.10221872756486959No Hit
GGATACTAACATCGGAATCGCAACTAACGATAACCAAAGAGAAGAAAGAA8910.10153499025674335No Hit
CTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATT8860.10096520916663818No Hit
TCTCAGTGCCAAGGAGGCACAGGATGTAATCATGGAAGTTGTTTTCCCTA8830.10062334051257507No Hit
CTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTCTT8800.10028147185851197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCC450.0128.96034145
ACACACC352.7523492E-7103.628845145
TTTCGCG554.1472958E-1092.32389145
GAGTTAA406.0966886E-790.67525145
CATGTGT259.365712E-487.04823145
TACTGGA259.4018783E-486.963832
GCACACC651.5570549E-978.12021145
TTCAGCG300.001931410272.5402145
CTTGGCC300.001931410272.5402145
AGGTGTG406.6444554E-572.5402145
ACCTACC651.3925091E-766.96018145
TAGGGGC1200.066.415555
TCACCGC702.3252687E-762.177307145
CCCTTCA350.00355853462.177307145
CTGATGG350.00355853462.177307145
AAATCGC606.782564E-660.450165145
TTAGGGG1350.059.036044
GACTATG1800.056.365442
CTAATTA651.0899477E-555.800148145
AGGGGCG1450.054.9645926
Produced by FastQC (version 0.11.5)