FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_Negative_Control_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_Negative_Control_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599738
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG805.8942715E-754.342139
TTACGGA850.002748125534.099874
TATACTG3204.1836756E-1127.1687975
GCGAAAG4150.026.195541
ACTAACG1701.0348605E-425.5727659
GCACCGT4750.024.4062886
GTAATAT2751.972603E-723.7188681
ACCGTGT5250.023.4619988
GTATTAA6050.022.760531
ATACTGC2552.7183141E-622.7313486
TCGCCTG1600.002182852222.6406635
CGAAAGC4505.456968E-1222.54382
GTGTATA2953.8822327E-722.110811
AGCACCG5250.022.0800365
TACACCG2003.115488E-421.7350355
GTATAAG2353.7161204E-521.5880951
CCGTGTT5151.8189894E-1221.1037399
GCCCTAT2455.123568E-520.7069471
ATACTGT5052.910383E-1120.0868596
ATTAGGG2556.979444E-519.891593
Produced by FastQC (version 0.11.5)