FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_Neg-Control-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_Neg-Control-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122672
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG13600.1211395670329357No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT12270.10929282996280303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4750.044.2442631
ATCGAAT1752.41198E-937.31306145
GCGAAAG5650.037.1882172
ACTGATC7650.035.0316478
TATACCG1251.2728109E-534.7696385
CGAAAGC6350.033.0828253
GTTACGT1652.3947305E-630.7444461
GAGCGAA2401.5897967E-930.1954361
GGTACTG7700.029.162855
GTACTGA8400.028.4534916
GCAGGTA7950.028.2520752
GGATAGC5050.027.253435
CAGGTAC8600.026.9544123
CTGATCC8600.026.9508089
AGGTACT8500.026.4181084
ATACCGA1400.001005422925.8668086
ATGTTGT19850.025.541036
CATGTTG13450.024.7739075
GCATGTT14750.024.554834
TAGCATG14900.024.3130532
Produced by FastQC (version 0.11.5)