FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1987_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1987_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582118
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGGGAGGATGACCAAGAAGCATGGGGCTGCGGTCTTTGGTATCAA8590.14756458312575801No Hit
TCCCTATCCTATGGGGAATTAATGGAAGATCCAGAACTAGAGCCTAGGTT8520.14636207779178792No Hit
TCTATGGGCAGTGTATTTTTTGCTACCCCAAGCTGCCTGCAGGCTTTCTG7240.12437340882776343No Hit
CAATATACAGATTACCTTTTTTTAAACTGGAGTTACCCAACAAGTGGACC7120.12231197111238616No Hit
TCACTAGACAGTGGCTATGGAGAAAAGTAGATGGAATCAAAAAATACTGA7090.12179661168354183No Hit
TGGTAGAGCAGGTGTTGAGGCACAAGGACACCAGCCAAGGGAGCAAACGA7030.12076589282585318No Hit
GTGCAGTGTGGTCAGTGACGCAGCACTCCGGGGAGAGATGGAGACGCAGA6460.11097406367781101No Hit
GTATAGTGGGGTCGGTGATAGTCTGCGTTAGATAGGGAATGATGTTTAGG6250.10736654767590076No Hit
GGATAATGGGTTGTGCCAGGTAACCAAAGCAGAATCCGTGATGGAAAATC5960.10238473986373896No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1550.088.8606341
GCGAAAG1850.074.43162
ATGGACG300.001940193872.454184
TGTACGC300.001940193872.454188
TATCCTA2000.072.454185
CGGATGC606.827573E-660.378489
TCACGGG450.00961209448.365154145
GCTACCG450.00961209448.365154145
GAGCGAA604.946197E-448.3277131
ACGGATG604.9587997E-448.3027848
CGAAAGC2900.047.469983
ATCCTAT3250.046.8165446
GCCTAAG2900.042.4950561
TATGTAC2150.040.4395456
CTATCCT3600.040.2523234
TATACTG1354.9912705E-737.5688325
CCTATGG3950.036.685668
GTATAGG800.00203707736.2457851
AAGGGTG2800.036.227095
TATAATA1458.7155786E-734.98692
Produced by FastQC (version 0.11.5)