FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1987_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1987_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940195
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAGACTGCTCAGGGTATAGCCCAAGGAACGGGGCTAAAGATAGAAG17860.18996059328118103No Hit
GTATATACTCAATAGGTAAGCTGAATGCTTACCTGCTTTATAAAGTCTGT12530.13327022585740192No Hit
ACTTATTACCGTCTTCAGAAAATCCAAGATATGGAAGGTGGAAGAAAGAA10960.11657156228229251No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3100.081.8186651
GAGCGAA1200.072.4679641
TAGACGT406.682866E-572.456394
CGAAAGC3650.069.482433
GCGAAAG4100.061.8563082
CGAAACA400.006065190354.3422934
TTACCGT2900.049.9672666
ACCGTCT3800.041.9462058
TATATAC6600.041.7195362
CTCAATA6600.040.617338
CGAATGA750.001478799738.690792145
ATTACCG3850.037.6356745
ACTCAAT7200.037.232557
CCGTCTT3900.037.1531649
GTATATA8200.037.1177371
TATTACC4100.037.1118134
CTAGACG800.002042041236.2301253
TACCGTC4100.035.3426977
ATACTCA8050.033.2993935
CGCCCGT1204.0734597E-430.1885578
Produced by FastQC (version 0.11.5)