FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1987_d02_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1987_d02_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959865
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC14540.1514796351570273No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11090.11553708073531173No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1751.010103E-733.1391941
TAATACG1153.1756924E-431.4985264
GTGATCG950.004745474630.5022459
GTTGACA10350.026.6154171
AGACGGT1450.00122160525.016823145
GTAGCAT14500.024.9972381
AGAGCGT1450.001232808524.9776786
GTACCTA4950.024.876766
TAGTACC3902.0008883E-1124.148874
TAGCATG13250.023.5171092
ACATGGT11500.023.3052645
GGTTATA3905.0386006E-1022.3052251
ACGTGTT2603.2244825E-622.2912654
GTATTAG3304.6948117E-821.9672681
GCTATAT2003.1052253E-421.7475971
GCATGTT15000.021.2510054
TGACATG14000.021.2165073
TTGACAT12750.021.0292072
ATAGGAC2806.1287847E-620.699033
GGTCCTA15700.020.3024489
Produced by FastQC (version 0.11.5)