FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1987-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1987-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050972
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAC1102.4472878E-432.929143
GTATAAT2259.127889E-725.7714061
ACCGTAG1500.001499800624.1514858
TAGTACC2751.9844447E-723.7078484
GTGTTAC5050.022.9646191
GATATAC1952.628546E-422.290493
TATACTG3604.8967195E-922.1335875
AGCGAAA5600.022.0034871
GTTATAC2353.7315403E-521.5790923
GCGAAAG6100.021.3881551
CTAGGAC4551.546141E-1020.6983153
ATACGTG1800.004327678620.1224026
GTATTAC4051.8875653E-819.6864911
CCGTAGA2659.3680006E-519.146211
ACACCGT5305.820766E-1119.1352636
GTATAGT2307.975543E-418.908371
GTCCTAG5107.075869E-1018.4758241
TAGTACT3201.941715E-518.1101634
CTATAAC3252.2178008E-517.8323943
CTAATAC2851.6427784E-417.7932873
Produced by FastQC (version 0.11.5)