FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1987-d10-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1987-d10-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321789
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT14360.10864063780225135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1753.7797945E-628.996081
ACCGCGC1450.00123223524.980538
CACCGTT2401.6096055E-624.1478467
GTACTAG3152.870911E-823.0127641
CTACACT7100.022.4481434
TACGGGG1952.6291818E-422.2903187
CCGCGCT2003.1201556E-421.733069
AACAGCG2053.6866465E-421.2029867
GCGAAAG5550.020.894122
ATACGAG2455.150886E-520.6958036
AGCGAAA5800.019.99731
TAATCGC1850.005028456419.60755145
TTACGTG2701.0821929E-418.7901952
CGAAAGC6201.8189894E-1218.6972313
ACCGTTC3201.9416666E-518.1108848
GTGTAGG10150.017.8547291
TACGTGT2901.8754607E-417.4883583
CTAGGAC11400.017.159633
CCGTTCA2550.001599679517.0455389
TACACCG3855.0136423E-616.9310055
Produced by FastQC (version 0.11.5)