FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1987-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1987-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888114
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT803.7356298E-545.3097841
GTGATCG1201.9854451E-742.2581339
ATACCGT800.00204556736.2171756
GCGAAAG3300.030.7453232
AGCGAAA3201.8189894E-1229.4513591
CGAAAGC3607.2759576E-1226.1612743
CTACTGA11450.024.0393496
TATACTA1851.8431207E-423.4895735
GCACCGT6750.022.5351316
GCCTTAA4607.2759576E-1222.0638961
TAGTACC3508.806819E-820.6978634
GTATTAT9550.020.4961531
AGTCCGC1800.004292728420.15019145
ACCGTCT1800.004326510220.1229238
CGTGTTG1800.00433216220.1183855
GGTTATA3651.3605677E-719.8618221
TAGAGTG5850.019.808875
AGCACCG7050.019.5191145
GTATACT1900.0059144119.063824
AGTATAC2308.000507E-418.8991153
Produced by FastQC (version 0.11.5)