FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1987-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1987-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592635
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGGACTGTGCAGTTTAAGGCAGTTAACTCTACCTTGAAACCCAAAG9330.15743248373788252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1000.072.464741
TAATTCG400.006077556454.3118254
CCTAGCG657.3670555E-444.574859
GCGAAAG1755.2750693E-1141.4049262
GTATAGC700.001061025541.3909263
GGACTAT1651.378794E-939.4995086
GCTAGGG1303.7115387E-739.016182
TACCGAT750.001489535238.6315357
GCACCGT1752.4538167E-937.2423936
CGAAAGC2002.2555469E-1036.217063
GACTATG1051.8627275E-434.492447
GCATATC1153.1491977E-431.541077145
GCCCGTT1153.1774445E-431.49317
ACCGATG1504.4230437E-528.973658
GAACCCC2502.4738256E-928.9663056
AACCCCG2354.3293767E-827.7407287
ATAGAGG2105.0203926E-727.5939523
AACGCCC2105.0203926E-727.5939527
TAGACTG1855.8636288E-627.400565
ACCCCGT2954.8748916E-1027.0093358
Produced by FastQC (version 0.11.5)