FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1986-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1986-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763422
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG24190.31686275742642994No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC20130.2636811619261693No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG13980.1831228337669074No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC13930.18246788800951505No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT13600.1781452460107254No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA13350.17487051722376354No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC11870.15548412280494928No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA11540.15116148080615963No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT10170.1332159670536086No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT9480.12417771560159388No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG8750.11461550754366524No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT8680.11369858348331591No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT8370.10963791978748319No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC7690.10073065748694693No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG1600.081.458815
GCAGGTA1700.076.7375642
AGGTACT1700.076.692264
GTACTGA2500.066.610836
ATGGGCG350.003560045362.169857145
TCCCGAT1400.062.0842134
GGGGTAT350.00358512762.0597846
TTCCCGA1550.056.0797423
CTTATAT701.6984923E-551.7708051
TATAGCG5700.049.5877272
AACACTG4550.049.332815
GCGAAAG901.3263852E-648.3162462
CCCGATT1850.046.9672435
GCGGTAT6150.045.914156
AAGTACC1307.365088E-944.602541
TATACTA4450.043.973212
GTATCCC6750.041.8658339
TTGATCG1053.8115159E-641.44657145
GTACCAC1401.4248144E-841.392193
TAGCACG907.5050746E-540.255619
Produced by FastQC (version 0.11.5)